The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols.

Fortessa Door Handles are covered by a 10 Year Guarantee on mechanical parts. Our levers have been tested to pass DIN EN 1906 European Quality Standards, and have been tested for frequency of use. Essentially each door handle is guaranteed for 100,000 revolutions.

Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3. Depressurize the sheath container by pulling up on the vent valve. 4. Remove the sheath container lid. 5.

The password is “BDIS”. 1.3. FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser.

2) Choose “New Experiment” .

The BD LSRFortessa™ cell analyzer offers the ultimate choice for flow cytometry. Designed for customization, it has the ability to meet the needs of your multicolor flow cytometry assays. The BD LSRFortessa has the flexibility to be configured with up to 7 lasers, which enables the detection of up to 18 parameters simultaneously.

Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. The BD LSRFortessa™ X-20 and BD FACSCelesta™ Operator Course Course Description This 4.5 days course teaches the operation of the BD LSRFortessa, BD LSRFortessa X-20 and BD FACSCelesta.

BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser. It is very similar in operation to the LSR II cytometers (RAL and ERML …

Be aware that the lasers take about 15 minutes to warm up and BD LSR Fortessa X20 – How to Use Guide 2 Section 1: Instrument Startup 1 The instrument is: OFF (go to step 2); ON (go to step 4). 2 Turn ON the cytometer pressing the green button on its right side. Confirm the computer was OFF before you do this.

In high- throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes. BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2.
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Table of Contents. 1. Computer and Software Login. 2.

Port (SIP). Page 4. Blizard Institute Flow Cytometry Core Facility.
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LSR II Quick Reference & Troubleshooting Guide Startup Ensure that you signed up for instrument use using the on-line calendar (even if it is night or weekend). Before switching on (or when starting your run): Check sheath reservoir. A full tank provides about 3 hours’ run time. Refill as necessary from large plastic canister containing 1x sheath solution. If machine is on, depressurize

Measuring Instruments BD FACSCalibur Instructions For Use Manual 238 pages. Flow cytometer.